Enzyme Catalysis Extended: Lipase-Catalysed Synthesis at ESS

After applying lactate dehydrogenase catalysis to the synthesis of perdeuterated enantiopure lactic acid, the ESS deuteration lab is now extending its use of enzyme catalysis to the lipase class of enzymes. The natural function of these enzymes is to hydrolyse ester bonds of triglycerides, releasing free fatty acids, under aqueous conditions. Lipases are also remarkably stable in organic solvents, and so can be encouraged to perform the reverse esterification reaction under water-free or water-limited conditions (Scheme 1).

lipase equilibrium reaction
Scheme 1. Lipase-catalysed hydrolysis/formation of esters.

Lipases are already used extensively in synthetic chemistry, so much so that many of them are commercially available in immobilised form. The ESS lab has recently procured a rotating bed reactor (RBR – Figure 1), and some lipases immobilised on various solid supports, contained within cartridges designed to be used in combination with the RBR. This equipment should allow the use of lipases to perform efficient synthetic transformations of deuterated molecules using immobilised enzymes.

Figure 1. The SpinChem® rotating bed reactor (RBR) system used for immobilised lipase reaction in the ESS deuteration laboratory.

Initial work has begun with a simple, unlabelled esterification system (Scheme 2). The reaction can easily be monitored by GC-FID, allowing for analysis of reaction progress and kinetics (Figure 2).

lauric acid esterification
Scheme 2. Lauric acid esterification catalysed by immobilised CalB lipase.
GC trace lauric acid est
Figure 2. Lauric acid esterification can be monitored by GC-FID, comparing the peak areas of the acid and ester in hexane.

Work will soon continue with more complex, deuterium-labelled systems to produce deuterated materials of use in neutron scattering experiments.

J-PARC Deuterated Materials Workshop

J-PARC (Japan Proton Accelerator Research Complex) last month held a workshop entitled “Deuterated Materials Enhancing Neutron Science for Structure Function Applications”, at the Ibaraki Quantum Beam Research Centre, Tokai, Japan. There were over 60 participants in the workshop, which was held over two days. J‑PARC have recently established on-site laboratories dedicated to chemical and biological deuteration and the aim of the workshop was to discuss how to activate deuteration science at J‑PARC.

JPARC workshop
The delegates of the “Deuterated Materials Enhancing Neutron Science for Structure Function Applications” workshop held at J-PARC in October 2017.

Three plenary speakers outlined the current status of other deuteration laboratories around the world. Dr Tamim Darwish and Dr Anthony Duff from ANSTO described the chemical and biological deuteration laboratories at the National Deuteration Facility, ANSTO, which have been functioning well as user laboratories for several years. Dr Anna Leung presented the research at the newly-established DEULAB at ESS, and also acted as a representative for the other members of the DEUNET (the laboratories at ISIS, ILL and FZJ). Professor Sajiki from Gifu Pharmaceutical University provided the fourth plenary session. Professor Sajiki has worked in the field of deuteration science for many years, and presented some of his recent results in the field.

Ten invited talks provided an excellent overview of the current research involving deuterated materials in Japan, and initiated excellent discussion about the future of deuterated materials research, and where more effort should be focussed to produce high-impact research. The meeting concluded that a deuteration community ought to be organised in Japan to leverage the advantages of collaborative research. Furthermore, this community ought to continue to interact with deuteration laboratories across the world. In addition to the existing collaboration between J‑PARC and the chemical deuteration laboratory at ANSTO, J‑PARC has joined the European chemical deuteration network (DEUNET) as an observer member, a development which is certain to benefit both deuteration communities and their users.

J-PARC joins the DEUNET


J-PARC (Japan Proton Accelerator Research Complex) is a multidisciplinary research facility with a series of proton accelerators producing neutron, pion, kaon and neutrino beams. This infrastructure is used to investigate research questions in areas including nuclear and particle physics and materials and life sciences. J‑PARC has recently built biology and chemistry laboratories in which research will focus on producing deuterated molecules for use in neutron experiments, and this month held a workshop entitled “Deuterated Materials Enhancing Neutron Science for Structure Function Applications”.

Japan has a strong reputation in the field of deuteration science, most notably in the form of Professor Sajiki at Gifu Pharmaceutical University. In Japan, a deuteration science community is beginning to organise around existing expertise, and new research which will be done at the J‑PARC deuteration laboratories. The chemical deuteration laboratory at J‑PARC has a strong collaborative project with the chemical deuteration laboratory at the National Deuteration Facility, ANSTO (AUS), with exciting developments being made in the area of highly and selectively deuterated ionic liquids.

The Deuteration Network members welcome J‑PARC to the DEUNET. We are certain this will bring benefits to all of the members and their respective user communities, and we welcome the exciting collaborations we anticipate in the near future.

Enzyme Catalysis for the Production of Perdeuterated Complex Molecules Established at ESS DEULAB

The chemical deuteration laboratory at ESS (DEULAB) is working to establish innovative methods of producing complex perdeuterated molecules with exciting applications in neutron scattering.

The first method focuses on the production of perdeuterated chiral molecules. Chiral molecules exist in two different forms (enantiomers) which are mirror images of each other but otherwise identical. The synthesis of a chiral molecule as a single enantiomer, without its mirror image, is challenging, and the inclusion of isotopic labelling imposes even more requirements upon equipment, methods and synthetic design. This is one reason why the number of perdeuterated chiral molecules available to the neutron scattering community is so limited.

Our method uses enzyme catalysts, which, due to the intricate design of their active site, often have full specificity for one enantiomer only, and can transform relatively economical, achiral precursors into valuable chiral molecules. Using deuterated analogues of  the precursors allows us to use this process to produce deuterium-labelled chiral molecules. Our first target molecule was lactic acid, which exists as both D-lactic acid, and L‑lactic acid (Figure 1). Lactic acid is best known as the molecule produced in the body during exercise, but it is also of interest in the fields of material science and biomedical technology as the monomer for poly(lactic acid) polymers which are renewable, biodegradable and biocompatible.

D and L lactic acid

Figure 1. The two enantiomers of lactic acid.

The lactate dehydrogenase enzyme is responsible for the production of lactic acid from pyruvate during normal metabolism and exercise, and normally it exists in only the L-form producing only L-lactic acid. However, D‑lactate dehydrogenase, which produces and acts exclusively on the other enantiomer D-lactic acid, has also been expressed recombinantly in bacterial hosts. We first used D‑lactate dehydrogenase to produce perdeuterated D-lactic acid-d4. In addition to the enzyme, we used two deuterated reagents, sodium pyruvate-d3, which we can easily synthesise in our lab and sodium formate-d1, which is commercially available. The other components of the catalytic system, including a buffer, a coenzyme, an acid and water as solvent – were all standard, unlabelled reagents. We isolated the product as D-lactic acid-d4 and analysed it using mass spectrometry to determine the level of deuterium incorporation (Figure 2). Pleasingly, we could see that the molecular weight of the product was four atomic mass units higher than for unlabelled lactic acid (one atomic mass unit per deuterium atom).

LA-d4 MS

Figure 2. Top: mass spectrum of unlabelled lactic acid (89.0 amu). Bottom: mass spectrum of D-lactic acid-d4; shifted by amu (93.0 amu).

This process is equally applicable to producing perdeuterated L-lactic acid-d4, by using L-lactate dehydrogenase as the catalyst. Both samples will be used by our Deuteration Network (DEUNET) partners at the Jülich Research Centre (FZJ) in Germany to synthesise tailored poly(lactic acid) samples, which will be analysed by neutron scattering to correlate the arrangement of the chiral monomers with the structure and physical properties of the polymers.

The number of similar enzymes which are commercially available, and the substrates they accept, suggests that this method will have broad applicability in the synthesis of chiral deuterated molecules, and so, at ESS, we are developing this enzyme-catalysis to produce more novel perdeuterated chiral molecules which are presently unavailable.

Deuteration for Neutron Scattering – DEUNET Workshop: Thanks for an Insightful and Inspiring Meeting!

From Monday 15th-Wednesday 17th May, the Deuteration for Neutron Scattering – DEUNET Workshop was held in Oxford, England, organised jointly by the STFC Deuteration Facility and the European Deuteration Network (DEUNET).

Prominent researchers working in the fields of lipids and membranes, energy materials, surfactants, polymers, protein structure and function, colloids, and other small molecules, presented their research which has exploited the power of the tandem techniques of deuterium-labelling and neutron scattering. The programme demonstrated the impact that deuterium labelling has on neutron science, and the ways in which this leads to high-impact science. Each of the facilities involved in the European Deuteration Network presented the current capabilities of their laboratories, in addition to projects and development being undertaken. It was very enjoyable to host delegates from other facilities around the world who work in the field of deuteration, such as J-PARC (Japan) and ORNL (USA), and to hear about the work undertaken in their laboratories.

There was a great deal of opportunity for delegates to interact outside of the presentations, during discussion sessions, a poster session, and within meal and coffee breaks. An advisory board was appointed for the DEUNET, comprised of Trevor Forsyth (ILL), Jian Lu (University of Manchester), Thomas Hellweg (Bielefeld University) and Peter Holden (ANSTO). We trust that they will succeed in providing the network with invaluable advice and insight.

We hope to hold the next STFC – DEUNET Deuteration for Neutron Scattering workshop in late 2018. Further information will be provided at Deuteration.net as it becomes available.

Thanks to all those involved in the workshop for a productive and inspiring meeting!

17EC1836_DEUNET Workshop

The delegates of the Deuteration for Neutron Scattering – DEUNET Workshop held in Oxford, 15-17th May 2017.

Deuteration for Neutron scattering – DEUNET workshop, Oxford 15-17 May 2017


We are pleased to announce the Deuteration for Neutron Scattering – DEUNET workshop, organised jointly by the STFC Deuteration facility and the DEUNET European Deuteration Network which will be held at the Oxford Spires Hotel, Oxford, UK 15-17th of May 2017.

Deuteration benefits neutron scattering investigations of many types of soft and biological material ranging from macromolecular structures to energy material function. The workshop aims to demonstrate the impact of deuterium-labelling in neutron science, and to establish applications in which this technique will play a key role in generating the greatest future scientific potential. The workshop will consist of a series of scientific presentations on the state-of-the art applications of neutron scattering and deuterium labelling and updates on the current capabilities of deuteration facilities in Europe and around the world. Extensive interactions between the delegates will be facilitate by dedicated discussion sessions in addition to a poster session.

Detailed information, including a preliminary program and list of confirmed speakers can be found at: https://indico.esss.lu.se/event/756/

Registration is now open and limited to 60 participants. Abstracts for posters are welcome until the 2nd April 2017.

We hope to see you in Oxford!

The organising committee:

Peixun Li, Marek Jura, John Webster STFC
Giovanna Fragneto ILL
Jürgen Allgaier FZJ
Hanna Wacklin ESS

Deuteration User Survey


Within the SINE2020 project we have setup an European Deuteration Network (DEUNET) that aims to increase the availability and accessibility of complex deuterated molecules to the European neutron scattering community. The existing capabilities of the laboratories at ISIS, ILL and FZJ which currently produce deuterated materials for neutron scattering will be complemented by an additional laboratory at ESS. Furthermore, the organisation of the facilities into a network leverages the unique specialisations of each of the laboratories.

Under this project we have prepared a short user survey to help us to get a better overview of your demands for deuterated materials. We hope you will participate in this survey.

Thank you for your help,